Basic Bruker Proton NMR Tutorial

Liquid Samples

This instrument is a 300 MHz Bruker Avance Digital FT-NMR capable of analyzing a variety of nuclei under a wide range of experimental conditions. A very brief list of capabilities includes 1D, 2D, T1, T2, COSY, SECSY, NOESY, INEPT, CP-MAS and HPDEC-MAS. Both solid and liquid probes can also be programmed for temperature controlled analyses. Data acquisition and processing software runs under an IRIS (Unix) X-Windows environment on a Silicon Graphics workstation computer. Most data files can be stored on Zip disk media.

The Bruker Avance 300 is a modern instrument utilizing superconducting magnet technology and interchangeable probes, allowing both liquid and solid samples to be analyzed with high resolution. Windowed software programs and a large selection of example experiments and pulse programs create a system that is easy enough to operate so that both instructors and students can rapidly obtain accurate proton spectra from liquid samples. These properties make it an excellent tool for teaching both the basic concepts of instrument operation and spectral interpretation, and the more advanced techniques involving 2D analyses.

Topics Outline:

1) Sample Preparation, Insertion and Removal

2) Instrument Startup, Optimization, and Shutdown

3) Running the Sample

4) Processing the Data

5) Plotting the Spectrum

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